Analyzed parameters

Sharpshooter species

 

O. facialis

A. citrina

D. costalimai

Frequency of Methylobacterium spp. isolation (G1)1

47

8

60.4

Frequency of Curtobacterium spp. isolation (G2)1

450

0.36

1.6

Percentage ofpositive specific PCR for Methylobacterium spp.2

51.7%

20.0%

8.7%

Percentage ofpositive specific PCR for Curtobacterium spp.2

89.6%

70.0%

39.1%

Number of isolates with ribotype I (Methylobacterium spp.) (out of 120)

10

1

0

Number of isolates with ribotype II (Curtobacterium spp.) (out of 120)

32

6

0

Transmission efficiency of Xfp
(according to Krügner et al., 2000)

1%

2%

5%
1Average of the number of bacteria isolated from each insect species in all sampling dates. CFU/head.
2Percentage of positive specific PCR for the detection of Curtobacterium or Methylobacterium using specific primers in a nested PCR. To each insect species 15 individuals were tested from each sampling date resulting in 45 insects tested.
Table 3: A comparative analysis of the Curtobacterium spp. and Methylobacterium spp. isolation and the efficiency of the sharpshooter species in transmitting Xylella fastidiosa subsp. pauca (Xfp), previously determined (Krügner et al., 2000).