Trichoderma spp. / Accession No. Protease (μg free amino acids.h-1.mg-1protein) 24 h 48 h 72 h 96 h Mean (T) T. harzianum (NBAII Th 1) 3.75 19.26 46.08 71.25 35.09 T. viride (NBAII Tv 23) 0.82 7.03 16.54 5.71 7.53 T. virens (NBAII Tvs 12) 38.60 29.52 54.26 60.22 45.65 T. koningii (MTCC 796) 39.94 20.15 38.84 25.86 31.20 T. pseudokoningii (MTCC 2048) 1.86 13.96 72.86 87.82 44.13 T. hamatum (NBAII Tha-1) 11.09 28.85 57.44 95.29 48.17 T. harzianum(Local) 4.83 33.27 40.91 62.96 35.49 M. phaseolina- Control 2.99 3.77 2.52 1.45 2.68 Mean (H) 12.98 19.48 41.18 51.32     S.Em. C.D. at 5% C.V.%     T 0.038 0.109 0.43     H 0.027 0.077     TxH 0.077 0.218     Values represent mean of three experiments, each performed in duplicate.

Table 3: Specific activity of protease produced by Trichoderma spp. incubated with cell wall of pathogen Macrophomina phaseolina.