Figure 1: ITS sequencing used to design primers specific for PCR amplification of potato soft rot pathogen rDNA. S52:Pectobacterium carotovorum subsp atrosepticum (Genbank, HM130661), S2: P. carotovorum subsp carotovorum (Genbank, HM130664), Pca1: Pectobacterium carotovorum subsp atrosepticum (Genbank, AF232687), Dch2: Dickeya chrysanthemi(Genbank, AF232681), Pca3: Pectobacterium carotovorum subsp atrosepticum (Genbank, AF373183), Pca4: Pectobacterium carotovorum subsp atrosepticum (Genbank, AF373178), Pca5: Pectobacterium carotovorum subsp atrosepticum (Genbank, AF373177), Pca6: Pectobacterium carotovorum subsp atrosepticum (Genbank, AF373179), Pca7: Pectobacterium carotovorum subsp atrosepticum (Genbank, AF373180), Pca8: Pectobacterium carotovorum subsp atrosepticum (Genbank, AF373181), Pca9: Pectobacterium carotovorum subsp atrosepticum (Genbank, AF373182), Pca10: Pectobacterium carotovorum subsp atrosepticum (Genbank, AF373176), Pcb11: Pectobacterium carotovorum subsp betavascolorum (Genbank, AF373184), Pcc12: Pectobacterium carotovorum subsp carotovorum (Genbank, AF373188), Pcc13: Pectobacterium carotovorum subsp carotovorum (Genbank, AF373189), Pcc14: Pectobacterium carotovorum subsp carotovorum (Genbank, AF373185), Pcc15: Pectobacterium carotovorum subsp carotovorum (Genbank, AF373186), Pcc16: Pectobacterium carotovorum subsp carotovorum (Genbank, AF373187), Dch17: Dickeya chrysanthemi (Genbank, AF373199), Dch18:Dickeya chrysanthemi (Genbank, AF373200), Dch19: Dickeya chrysanthemi (Genbank, AF373201), Dch20: Dickeya chrysanthemi (Genbank, AF373202), Dch21: Dickeya chrysanthemi (Genbank, AF373203).Sequences of the primers are shaded and arrows indicate the direction of the priming of the primers.