Primer sequence Polymorphic bands Total bands Polymorphism % The size of band (bp)
OPA-01 5´-CAGGCCCTTC-3´ 8 9 88 200 - 1000
OPA-02 5´-TGCCGAGCTG-3´ 3 5 60 100 - 700
OPA-03 5´-AGTCAGCCAC-3´ - - - -
OPA-04 5´-AATCGGGCTG-3´ - - - -
OPA-05 5´-AGGGGTCTTG-3´ - - - -
OPA-06 5´-GGTCCCTGAC-3´ - - - -
OPA-07 5´-GAAACGGGTG-3´ - - - -
OPA-08 5´-GTGACGTAGG-3´ 3 4 75 100 - 700
OPA-09 5´-GGGTAACGCC-3´ 3 5 60 100 - 1500
OPA-10 5´-GTGATCGCAG-3´ 4 5 80 100 - 1000
OPA-11 5´-CAATCGCCGT-3´ 5 6 83 100 - 1200
OPA-12 5´-TCGGCGATAT-3´ - - - -
OPA-13 5´-CAGCACCCAC-3´ 4 4 100 100 - 700
Table 3: Primers used in this study and major random amplified polymorphic DNA fragments generated from the F. oxysporum isolates.