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Figure 1: Differential display using chitinase-based primer (A: Chi 15, B: Chi 25, C: EZ, D: EzA1A13, E: PR1 and F: PR2). M: 10 Kbp DNA marker, C: control, 1: F+B, Lane 2: F+T, Lane 3: R+B, lane 4: R+T. The first four samples collected after 2 hours, the second four samples collected after 4 hours, the third four samples collected after 6 hrs, the fourth four samples collected after 8 hrs, and the fifth four samples collected after 24 hrs. Numbers reefer to the isolated and identified genes (1, Amino Acid/Auxin Permease; 2, Endopolygalacturonase PG; 3, Fructose-1,6 bisphosphatase; 4, Glycosyl transferase group 1; 5, Protein tyrosine kinase). (F=F. oxysporum; R=Rhizoctonia solani; B=Bacillus subtilis; T=Trichoderma viride). |