|
Primer |
Primer sequence 3̀→5̀ |
Annealing °C |
Real time PCR |
Chitinase1 Forward |
TTC GTG GAG TGC ATC GGT |
60 |
Chitinase1 Reverse |
TAN GCN CNN NCC NGG RTT |
Defensin 1 Forward |
CAA TGT AAC TTA AAG TGC CTA ATT ATG |
55 |
Defensin 1 Reverse |
CTT ATC AGA TCT CAA TGG AGA AAT C |
Defnsin 2 Forward |
TCA CCA AAC TAT TGG ATT TCA A |
57 |
Defensin 2 Reverse |
GAC TCA ATT TTT GAC TTC TTA ATCC |
Differential PCR |
EZ |
GCATCACAGACCTGTTATTGCCTC |
45 |
Chi15 |
GGYGGYTGGAATGARGG |
55 |
EzA1A13 |
CAG GCC CTT CCA GCA CCCAC |
53 |
Chi 25 |
GAYTTRTGGGAATAY CC |
52 |
PR1 |
CCCCAAGCTTTTAGTATGGCTTCTCGTTCACATAAT |
40 |
PR2 |
CGCGGATCCTACTGGCTATTCTCGATTTTTAATCG |
40 |