Assay Primer Type Position on gene Length Sequence(5´-3´) PCR F Forward 1698-1717 20mer GATTCGCCTTGGTCGAAATA B Backward 1908-1927 20mer GGTTTGTCCTCACCCTTTCA LAMP F3 Forward outer 1014-1031 18mer GCCTGGATTCTGGTGTGG B3 Backward outer 1169-1186 18mer CCGTCCCAGCCAAGATCA FIP(Forward inner primers) F1c (Forward inner) 1077-1099 23mer TACCAGACGTCTGGCTACCCTGT F2 (Forward inner) 1033-1051 19mer GTAGGGTAGGGTAGGCGTA BIP(Backward  inner primers) B1c (Backward  inner) 1005-1024 20mer ATGGGGGTGTAGGGTAGGTC B2 (Backward  inner) 1047-1067 22mer TCCTCCCTCGACTAAAGCGAAG

Table 1: Oligonucleotide primers used for LAMP and PCR based on 28s ribosomal RNA gene of Fusarium oxysporum f. sp. lycopersici.