Figure 4: Distribution and tissue subcellular localisation of GR protein within human myometrium by fluorescence confocal microscopy. GR protein localisation and expression in non-pregnant (A) and pregnant non-labouring sections of human myometrium (upper uterine sections are shown in B; lower segment pregnant non-labouring sections not shown). The left panels show GR protein staining visualised using a FITC conjugated secondary antibody (stains green). Nuclear DNA was defined by counterstaining with DAPI (stains blue) as shown in the middle panels. Colocalisation is illustrated by merging the images as shown in the right panels. Corresponding control reactions for each of the tissue sections were negative as they showed no immunofluorescence staining above background (not shown).