Figure 2: Effect of addition of Progesterone on SLPI expression in BeWo cells. A & B: Time course and C&D: Specificity of progesterone action. A) Cells incubated with or without P4 (1 M) for 024 h. RNA isolated reverse transcribed cDNA was subjected to semi-quantitative PCR in the linear range of amplification with GAPDH as an internal control. B) Graphical representation of results presented in A, (values expressed as fold increase over control mean S.E. from three independent experiments). C) RT-PCR analysis for expression of SLPI 1) control, (2) P4 (1M), (3) RU486 (1M), (4) P4 + RU486 (both at 1M) and incubations were carried out for 24 h. RNA was reverse transcribed and cDNA was subjected to semi-quantitative PCR in the linear range of amplification with GAPDH as an internal control. D) Graphical representation of results presented in C (values expressed as fold increase over control; mean S.E. from three independent experiments).
* Significantly different from group 2 p<0.001.