Temperature (°C) 40.0 37.5 30.0 25.0 No. of experiments 7 8 6 8 Slope of 1st Isoprenaline Curve 0.92 ± 0.06 1.02 ± 0.03 0.83 ± 0.03 0.95 ± 0.04 Slope of 2nd Isoprenaline Curve 0.82 ± 0.03 1.03 ± 0.05 0.81 ± 0.03 1.02 ± 0.08 0.17 ± 0.03 0.095 ± 0.04 0.06 ± 0.05 0.02 ± 0.06

Table 1: Mean Slopes of Linear Fitting for the Hyperbolic Langmuir Model to 1st and 2nd Isoprenaline Dose-Response Curves and Mean Corrections for Changes in EC50 between the two Curves.