parameters

First binding sites

Second binding sites

       p

1

1

        gi

6.00±0.21

8.00±0.27

        Ka / M-1

547198.10±38.00

482163.14±22.18

ΔH/ kJ mol-1

-10.57±0.08

-13.30±0.06

ΔG/ kJ mol-1

-34.02±0.11

-33.70±0.14

ΔS / kJ mol-1K-1

0.056±0.002

0.066±0.003

-0.27±0.08

 

 

2.72±0.12

Table 3:Binding parameters for ASP+HSA interaction recovered from Eqs.1 and
2 at pH 7 and 310K. p=1 indicates that the binding is non-cooperative in the two
sets of binding sites. The positive value of indicate that ASP+HSA complex
is stable and the negative value of imply that aspirin destabilizes the HSA
structure in the low aspirin concentration region. The interaction of aspirin with
HSA at 310K is weaker than that of at 298K as indicated by smaller equilibrium
association constants.