| Table 1: Immunocytochemistry of ADAS cells to assess neuronal and glial
markers. Non-induced - ADAS cells grown under control conditions in DMEM,
10% FBS, 1% streptomycin-penicillin and 0.1% gentamycin. Induced - ADAS cells
after 24 h culture in neuronal induction medium. Diaminobenzidine (DAB) served as
a brown chromagen. The percentage of cells showing specific immunolabeling was
quantified in three randomly selected fields at 200X with a bright field microscope.
The number of cells positive for immunolabeling was divided by the total number
of cells in the same field as determined by phase optics to obtain the percentage
of cells that were immunolabeled. All images were obtained at 200X magnification.
NESTIN (marker of immature neural cells), GFAP (mature glial cells - astrocyte),
NSE (early neuronal) e S100 (nuclei of astrocytes and Schwann cells). |
(glial fibrillary acidic protein), NSE (neuron-specific enolase)