Reaction step |
Temp (°C) |
Time (sec) |
Cycles |
Analysis mode |
Acquisition mode |
Hot start |
95 |
60 |
1 |
None |
None |
Amplification |
95 |
5 |
55 |
Quantification |
None |
59 |
5 |
None |
72 |
10 |
Single |
Melting curves |
55 to 95 |
20°C/sec (Ramp) |
Melting curves |
Continuous |
Cooling |
To 40 |
30 |
1 |
None |
None |
|
