Example of growth curves of ORS cells cultivated according to the current
method (▲), Dieckmann explant method (●), Dieckmann explant method with
50 μg/ml Geneticine supplement over 48 h at early passages of adherent
culture (*). The lower part of the graph is displayed linearly and the upper
part logarithmically. The cells cultivated upon the method described herein
reached the number of 1,000,000 cells by the second passage and as much
as 80,000,000 cells by the passage 5 of adherent culture (displayed results of
single donor using comparative methods). |
