Figure 2: (A) Dose-dependent binding between Ab3 (anti-anti-id) sera from three immunized mice or RP215 and microwell-coated CA215. Alkaline phosphatase-labeled goat anti-mouse IgG was used as the secondary antibody for signal detection at 405nm. (♦), (■) and (▲) denote Ab3 sera from three BALB/c mice (designated as L, R and I) immunized with rat anti-id Mab, respectively (initial dilution 1:200). (●) and (×) denote RP215 and normal mouse IgG (initial concentration = 1µg/mL), respectively. (B) Western blot assay to compare molecular weight of the protein bands detected in OC-3- VGH cancer cell extract using RP215 or Ab3 sera as the probe with reducing and non-reducing SDS-PAGE. Molecular markers of 60 KDa and 160 KDa were shown on the left. From left to right: Lane 1: RP215; Lanes 2, 3 and 4 are Ab3 sera from three immunized mice designated as I, R and L, respectively (concentration = 1:10,000 dilution); Lane 5 is negative control with normal mouse sera of the same dilution.