Animals

Mean OD (± SD) at 450 nm

Results

Cat 3

0.43 (± 0.001)

Positive

Cat 4

0.51 (± 0.02)

Positive

Cat 5

0.38 (± 0.02)

Positive

Cat 1 (control, non fed)

0.25 (± 0.03)

Negative

Cat 2 (control, non fed)

0.27 (± 0.02)

Negative
OD = optical density; SD = standard deviation
The cut-off point = 0.35, was calculated as a mean of the negative controls + (2 x SD). Mean values were calculated from the absorbance values of triplicate samples.
Table 3: Results of detection of anti-anti-gp120 in the Cats’ serum by ELISA