Rabbit (JW)

peptide vaccine

Inoculum (IV)EBV (B95-8)

EBV-DNA quantity in blood (copies/106 cells)

maximum EBV-DNA in blood (copies/106 cells)

Increase in anti-EBV antibody titer

observation period (days)

 EBER-1 expression* in necropsy materials

N-36

−a

9.2×107 copies

9.4×101 (4 days)

6.6×104 (9 days)

ND

28, killed

positive** (++)

N-38

 9.2×106 copies

1.8×101 (4 days)

9.6×102 (8 days)

ND

28, killed

positive (++)

N-82

9.2×105 copies

1.7×104 (14 days)

1.7×104 (14 days)

ND

49, died

positive (+)

N-83

 9.2×104 copies

5.3×101 (21 days)

1.3×103 (28 days)

+ (EA-IgG), − (VCA-IgM, VCA-IgG, EBNA-IgG)

91, killed

positive (+), a few

N-84

 9.2×104 copies

negative

negative

− (VCA-IgM, VCA-IgG, EBNA-IgG, EA-IgG)

70, killed

positive (+)

P-204

9.2×104 copies

negative

negative

ND

70, killed

positive (+)

P-206

9.2×104 copies

negative

negative

ND

70, killed

positive (+),  a few

O-19

 +

9.2×104 copies

1.3×103 (35 days)

1.3×103 (35 days)

− (VCA-IgM, VCA-IgG, EBNA-IgG, EA-IgG)

49, died

positive (+++)

O-21

 +

9.2×104 copies

negative

negative

− (VCA-IgM, VCA-IgG, EBNA-IgG, EA-IgG)

70, killed

negative

O-22

 +

9.2×104 copies

negative

negative

ND

42, died

positive (+), few

L-143

 +

9.2×104 copies

negative

negative

− (VCA-IgM, VCA-IgG, EBNA-IgG, EA-IgG)

70, killed

positive (+), a few

O-208

 +

9.2×104 copies

negative

negative

ND

119, killed

positive (+), few

O-209

 +

9.2×104 copies

negative

negative

ND

119, killed

positive (+), few

VCA: viral capsid antigen; JW: Japanese White; EBNA: EBV-associated nuclear antigen; EA: early antigen; ND: not determined

 

*: EBV-encoded RNA expression examined by in situ hybridization (ISH); **: EBER-1-positive lymphocytes
EBER-1 positive: (+++), more than 15%; (++), 5% – 15%; (+), 1% –less than 5%; (+) a few, 3 positive cells– less than 1%/ section; (+) few, 1 – 2 positive cells/section.

a:indicates treatment with keyhole-limpet hemocyanin and adjuvant without peptides before EBV inoculation.

Table 1: Summary of Vaccination Studies with Synthetic Peptides of EBV-major envelope glycoprotein-350/220 using a JW Rabbit EBV Infection Model.