Figure 6: Preparation of VLPV expressing JUNV and MACV GPC Genes.
(A) Bi-cistronic vector and packaging helpers were derived from the TC-83 IND vaccine. The human codon-optimized JUNV GPC and MACV GPC genes were cloned into TC-83 vector downstream from the TC-83 subgenomic 26S promoters as shown. (B) Preparation of VLPVs was carried out in BHK-21 cells co-transfected with bi-cistronic vector and two helper RNAs.