BALB/c mice (n=5 per group) were immunized i.n./i.m. with FPV-HIV/VV-HIV (grey bar) and FPV-HIV IL-13Rα2/VV-HIV IL-13Rα2 (black bar). At 3 days (acute stage), 14 days (effector stage) and 8 weeks (memory stage) post-boost immunization, (A) percentages of KdGag197-205- positive CD8+ T cells were measured by tetramer staining following FACS analysis. Following post-boost immunization, splenocytes were stimulated with KdGag197-205 peptide for 20 hrs and the percentages of KdGag197-205- specific (B) CD8+IFN-γ+ (C) CD8+ IL-3+ (D) CD8+IFN-γ+IL-3+ and (E) CD8+GM-CSF+ T cells were measured by intracellular cytokine staining. Unstimulated cells from each sample were used as a background control and this value was subtracted from each sample before plotting the data. These experiments were repeated over three times and in each graph the data represent mean of three experiments (n=15/group) plus standard error of the mean. p values were calculated using two-tailed unpaired student’s t-test.
