Figure 2: (A) Analysis of purified HA-NA-M1 Vlp by Western blotting using chicken serum DK/Hok/Vac-1/04 specific for H5N1 (upper panel) and rabbit monoclonal anti-neuraminidase antibody (bottom panel). Lane 1: Marker; Lane 2: Positive control, HA; Lane 3: Positive control, NA; Lane 4: Positive control, M1. Lane 5: HA-NA-M1 VLP. (B) Negative staining electron microscopy of H5N1 influenza Vlps comprising the HA, NA, and M1 proteins. Bars represent 100 nm.