Figure 1: Periodate-treatment of F. hepatica total lysate (FhPox) enables the production of IFNγ by splenocytes from infected animals. A) Splenocytes from infected or naïve animals were stimulated in vitro with total lysate FhTE (75 g/ml) for 72 h at 37°C. IFNγ and IL-5 were detected in culture supernatants by specific ELISA. B) Splenocytes from infected or naïve animals were stimulated in vitro with total lysate FhTE (75 g/ml), periodate oxidized-lysate (FhPox, 75 g/ml)) in absence or presence of LPS and incubated for 72 h at 37°C. IFNγ and IL-5 were detected in culture supernatants by specific ELISA. Controls consisted in the FhTE lysate incubated only with borhydrure (FhB), or PBS subjected to the complete periodate treatment (CPox). Results are expressed as mean value of triplicates (± SD, indicated by error bars), and are representative of three different experiments. Asterisks represent differences statistically significant (*p <0.01).