Figure 4: Polymorphism in DNA electrophoretic pattern following PstI and TaqI endonuclease digestion of PCR-amplified MHC class II gene of rural and reference Saanen goat sperms
Lane 1: 100 bp molecular marker
Lane 2: negative control
Lanes 3-6: PstI digested amplicons pattern of 4 respective rural goats’ MHC class II gene
Lane 7: PstI digested amplicon of reference Saanen goat MHC class II gene
Lanes 8-11: TaqI digested amplicons pattern of 4 respective rural goats’ MHC class II gene
Lane 12 : TaqI digested amplicon of reference Saanen goat’s MHC class II gene.