Table 1

Time (min)	0.1% TFA in water	ACN	Mode
→ 7.0	70 → 10	30 → 90	Linear gradient
7.0 → 10.0	10 → 0	90 → 100	Linear gradient
10.0 → 13.0	0	100	Isocratic
13.0 → 13.01	0 → 70	100 → 30	Sharp gradient
13.01 → 17.00	70	30	Reequilibrium

Table 1: Optimized HPLC method for assessing imipramine in in vitro samples.