Figure 12: NANOG gene expression in the ECO cells. The RNA from the ECO cells was reverse transcribed into cDNA, which was then amplified by polymerase chain reaction with the primers for NANOG. Labels: ECO – patients’ samples (encoded A-F); ESC – culture of the human embryonic stem cells H7 (G); ECT -culture of the human testicular embryonal carcinoma cells NT2D1 (H); PBMC – peripheral blood mononuclear cells; BMMC – bone marrow mononuclear cells (no amplicons - empty lanes not shown). Intentionally, the entire lanes are shown to demonstrate specificity of amplification with the entire absence of mispriming. All the gels of amplicons were normalized and calculated with the statistical significance at the P < 0.001. The samples were run in triplicates. The samples are representative for all amplifications.