Symbol

 

Description

ALDHbr

ALDHdim

ALDHbr vs. ALDHdim

mean ΔCt

SD

mean ΔCt

SD

Fold change

p value

Most highly expressed genes in ALDHbr cells

ENG

CD105 [endoglin]

4.31

0.95

10.38

1.44

66.93

0.04

TIMP1

TIMP metallopeptidase inhibitor 1

4.78

0.23

5.26

0.11

1.39

0.03

IL8

Interleukin 8

4.79

1.59

2.11

0.20

-6.38

<0.001

TGFB1

Transforming growth factor, beta 1

5.39

0.52

4.05

0.59

-2.54

0.03

EREG

Epiregulin

5.41

0.90

6.11

0.27

1.63

0.20

EPHB4

EPH receptor B4

5.94

0.59

11.96

0.23

64.60

0.01

AKT1

V-akt murine thymoma viral oncogene homolog 1

6.02

0.76

7.10

0.13

2.11

0.10

HIF1A

Hypoxia inducible factor 1, alpha subunit

6.60

0.51

5.44

0.23

-2.24

0.01

SPHK1

Sphingosine kinase 1

7.06

0.78

10.28

0.22

9.30

0.01

COL18A1

Collagen, type XVIII, alpha 1

7.30

0.91

10.76

0.73

10.97

0.03

VEGFA

Vascular endothelial growth factor A

7.39

0.84

7.91

0.03

1.43

0.24

TIMP2

TIMP metallopeptidase inhibitor 2

8.00

1.26

6.02

0.82

-3.95

0.08

ANPEP

Alanyl (membrane) aminopeptidase

8.36

1.85

7.21

1.00

-2.23

0.28

S1PR1

Sphingosine-1-phosphate receptor 1

8.53

0.39

4.81

0.70

-13.15

0.01

FGFR3

Fibroblast growth factor receptor 3

8.80

0.62

12.25

0.43

10.87

0.01

MMP2

Matrix metallopeptidase 2 (Collagenase)

8.83

1.43

13.31

0.27

22.44

0.07

MDK

Midkine (neurite growth-promoting factor 2)

9.03

1.21

11.13

0.64

4.28

0.11

MMP9

Matrix metallopeptidase 9 (Collagenase)

9.18

0.92

2.44

0.94

-106.64

0.02

ID1

Inhibitor of DNA binding 1

9.33

0.39

8.49

0.33

-1.79

0.02

PTGS1

Prostaglandin-endoperoxide synthase 1

9.40

0.54

12.21

0.74

7.01

0.02

PROK2

Prokineticin 2

9.45

2.37

7.44

1.44

-4.02

0.33

TYMP

Thymidine phosphorylase

9.50

0.38

6.62

0.67

-7.34

0.01

SERPINF1

EDG1

9.61

0.77

9.86

0.56

1.19

0.55

IL1B

Interleukin 1, beta

9.63

1.44

6.83

0.46

-6.97

<0.001

PECAM1

CD31

9.69

2.57

7.90

6.91

-3.45

0.29

Other genes differentially expressed in ALDbr vs. ALDHdim cells

TEK

TEK tyrosine kinase, endothelial

10.57

1.12

13.32

0.52

6.75

0.14

TNFAIP2

Tumor necrosis factor, alpha-induced protein 2

10.78

0.62

8.53

0.19

-4.77

<0.001

CXCL1

Chemokine (C-X-C motif) ligand 1

10.82

1.77

7.64

1.42

-9.10

0.11

THBS1

Thrombospondin 1

10.97

1.69

8.24

1.18

-6.64

0.05

ID3

Inhibitor of DNA binding 3

10.99

0.49

7.51

0.16

-11.14

<0.001

ANGPT1

Angiopoietin 1

11.15

0.81

13.94

0.90

6.96

0.03

TNF

Tumor necrosis factor

11.76

1.22

6.17

0.74

-48.27

0.01

EFNB2

Ephrin-B2

12.66

0.95

11.10

0.41

-2.94

0.03

VEGFC

Vascular endothelial growth factor C

12.99

1.96

10.19

5.73

-6.96

0.29

IL6

Interleukin 6 (interferon, beta 2)

13.96

0.45

9.22

0.96

-26.86

0.04

IFNG

Interferon, gamma

13.99

0.92

7.66

0.89

-80.49

0.01

PLXDC1

Plexin domain containing 1

14.00

1.12

11.23

1.77

-6.83

0.13

CXCL6

Chemokine (C-X-C motif) ligand 6

14.69

0.70

10.38

1.08

-19.87

0.05

KDR

Kinase insert domain receptor/VEGF receptor

15.08

1.22

13.24

0.61

-3.60

0.03

COL4A3

Collagen, type IV, alpha 3

15.19

1.22

13.55

0.44

-3.10

0.04

HAND2

Heart and neural crest derivatives expressed 2

15.19

1.22

13.30

0.54

-3.70

0.02

Table 1: Expression of angiogenic genes by ALDHbr and ALDHdim cell populations of human bone marrow cells. Top section. QRT-PCR based expression data for the 25 gene products most highly expressed in ALDHbr cells are listed in order from the most to least abundant transcript. The Fold Change columns shows the fold up-regulation [positive numbers, red] or down regulation [negative numbers, blue] of expression of ALDHbr cells compared to ALDHdim cells. The last column indicates the p-value [t-test] for the fold regulation of expression between the populations. Bottom Section. Expression data for other genes selected as described in the text.