ALDHbr vs. Hypoxic ALDHbr in monoculture

ALDHbr vs. Hypoxic ALDHbr with HUVEC in transwells

Gene

Fold Regulation

p value

Fold Regulation

p value

Genes significantly upregulated under both culture conditions

CXCL5

16.0

<0.001

140.3

0.02

EFNA1

11.2

<0.01

7.2

0.04

CXCL6

11.0

 0.01

11.6

0.03

TIMP3

10.0

<0.01

20.3

0.02

COL4A3

9.1

<0.01

15.6

0.02

PLAU

9.1

0.03

6.0

0.01

FGF1

6.7

<0.001

22.6

0.02

PLG

6.7

<0.001

24.3

0.02

LEP

6.4

<0.001

23.7

0.02

CCL11

5.8

<0.001

22.2

0.02

HAND2

5.6

<0.001

21.9

0.02

PGF

3.6

0.0376

18.5

0.01

Genes significantly regulated in hypoxic monoculture only

IL1B

6.4

0.03

   

KDR

4.7

0.04

   

TIMP2

4.2

0.03

   

CXCL9

3.7

0.01

   

JAG1

3.3

0.01

   

S1PR1

3.1

0.04

   

ID1

1.8

0.02

   

COL18A1

-9.2

0.03

   

Genes significantly upregulated in hypoxic cultures with HUVEC only

IFNA1

   

16.1

0.02

EFNA3

   

11.9

0.03

IL6

   

9.8

0.02

ANGPTL3

   

8.1

0.02

CCL2

   

7.7

0.04

EGF

   

7.4

0.04

THBS2

   

6.9

0.03

SPHK1

   

3.5

<0.01

Table 3: Expression of angiogenesis genes by freshly isolated ALDHbr cells and ALDHbr cells cultured in transwells under hypoxic conditions with and without HUVEC networks. Cultures were maintained as described in the text. Values are fold change in mean expression of genes in the angiogenic array that were significantly (p<0.05) upregulated (positive values) or down regulated (negative values). Gene expression was measured by QRT-PCR.