Usage

Primers Used (5’-3’)

Amplimer
size

Promoter
-Reporter

hsNgProm -2401-AttB1-F1:
ggggACAAGTTTGTACAAAAAAGCAGGCTTAtctctcatgcctttacccaaa

hsNgProm -1817-AttB1-F1:
ggggACAAGTTTGTACAAAAAAGCAGGCTTaaaaatctaaagtcagatagctt
cc

hsNgProm -1788-AttB1-F1:
ggggACAAGTTTGTACAAAAAAGCAGGCTTAcctcaactttattccaattgctt
t

hsNgProm -1737-AttB2-R1:
ggggACCACTTTGTACAAGAAAGCTGGGTAggccgacttactacattcttcg

hsNgProm -1702-AttB2-R1:
ggggACCACTTTGTACAAGAAAGCTGGGTAgtgaaagaccaaagggaagg

hsNgProm -258-AttB1-F1:
ggggACAAGTTTGTACAAAAAAGCAGGCTTAtcccattcctgttgaaccat

hsNgProm +34-AttB2-R1:
ggggACCACTTTGTACAAGAAAGCTGGGTAaacgttaaaatcctggagtctct

Hoxb6 Prom-AttB1-F1:
ggggACAAGTTTGTACAAAAAAGCAGGCTTaggttgataggtttgtgcgc

Hoxb6 Prom-AttB2-R1:
ggggACCACTTTGTACAAGAAAGCTGGGTAccgggtttatgatttgttgtgt

 

qRTPCR

NANOG F3: tgtgggcctgaagaaaactatc
NANOG R3: gctgtcctgaataagcagatcc
NANOG F1: tccctttggtctttcactcc
NANOG R1: ctccctgtcccattgtgtct
NANOG F2: tatgcaaagacccccttctg
NANOG R2: gctctccaaagggcaggta
GAPDH F1: tgggtgtgaaccatgagaagta
GAPDH R1: gagtccttccacgataccaaag

111bp

55bp

58bp

125bp

Table 1: Primers used in the Study. For the following promoter primers the attB site sequences are shown in capital letters. Information for EMSA primers can be found in Figure 5.