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Figure 2: RT-PCR and real-time RT-PCR verification of the microarray data. A. Loss of expression of Hoxa10 in Hoxa10-mutant is confirmed by RT-PCR and qRT-PCR. B. qRT-PCR of selected genes on 4.5dpc Hoxa10-/- and +/- uterine tissue. mRNA level was normalized to that of housekeeping gene, ribosomal protein L7 (Rpl7). C. RT-PCR results of some DRGs on 4.5dpc control and Hoxa10-/- uteri. * denotes P<0.01, and **denotes P<0.05, when compared to Hoxa10-/-. |