Ave. percentage of successful implantation
mean ± sdv %
Control*
8/8
49
39
81.48 ± 17.63
Hoxa10-/-**
2/5
30
3
7.78 ± 10.83***
Hoxa10-/-; Rag1-/-
2/8
47
3
5.21 ± 9.90***
Blastocysts collected from 3.5dpc naturally-pregnant wild-type females were transferred to the distal uterine horn of 2.5dpc pseudo-pregnant recipient females, bypassing
the homeotic-transformed region of the mutant uteri. 5 or 6 days later, recipients were sacrificed and uteri examined to determine the development of embryos. Successful
implantation was defined as normal embryonic size and absence of hemorrhage at the above mentioned time points, *Control group included 1 wild-type, 5 Hoxa10+/-Rag1+/- and 2 Hoxa10+/-;Rag1-/-mice. **Hoxa10-/- group included 3 Hoxa10-/- and 2 Hoxa10-/-; Rag1+/- mice. *** denotes P<0.0001 compared to control.
Table 2: Embryonic survival following blastocyst transfer to control, Hoxa10-/- and Hoxa10-/-;Rag1-/- pseudo-pregnant females.