Figure 5: Localization of Flt4 mRNA in non-implantation (NIS) and implantation (IS) tissue segments from Day 5.5-9.5 pregnant mice. (A-B) Day 4.5 NIS, (C-E) Day 4.5 IS, (F-H) Day 7.5 IS, (I-L) Day 8.5 IS and and(M-P) Day 9.5 IS. These are representative of at least 3 independent samples. Global linear adjustments of the brightness and color level were made on the photomicrographs to more accurately represent what was seen on the slides under the microscope. Numbers above scale bars are in microns. Hybridization signal is purple while nuclear stain is pink. All sections in photomicrographs are oriented mesometrial (MM) and anti-mesometrial (AM) regions down and up, respectively. Abbrev: c, conceptus; cm, circular smooth muscle; ec, endothelial cell; Endo, endometrium; lm, longitudinal smooth muscle; Myo, myometrium; pdz, primary decidual zone; sa, spiral arteriole; tgc, trophoblast giant cell.