Figure 1: OpNS-EM images of CETP(10) and IgG antibody(6). OpNS can be used as a general protocol for non-lipoprotein imaging. One example is a structure-known small protein, 53kDa CETP. (A) OpNS images shows worm-shape of CETP, (B) selected raw particles and (C) reference-free class average displayed a near perfect fit (D) to crystal structure in shape. Another example is a structural semi-known small protein, 160kDa IgG antibody, (E) OpNS images shows “Y-shape” structure of antibody. Selected raw particles (F) display three domain detail structure that are consistent to the crystal structure of antibody (G) (PDB 1igT), including the hole in domain. Scale bars: A and E, 50 nm; B-D, F, and G, 10 nm.