Ionic strength (mM KCl)
From M 540 fluorescence quenching,
K
m
× 10
-4
, (М
-1
)
From 1,8 ANS fluorescence quenching,
K
m
× 10
-4
, (М
-1
)
рН 6.4
рН 7.4
рН 6.4
рН 7.4
0.01
0.05
0.1
0.15
3.0 ± 0.3
2.2 ± 0.2
1.3 ± 0.2
1.1 ± 0.2
3.1 ± 0.3
2.1 ± 0.2
1.25 ± 0.2
1.0 ± 0.2
3.8 ± 0.4
2.5 ± 0.3
1.75 ± 0.2
1.2 ± 0.2
3.5 ± 0.4
2.8 ± 0.3
1.8 ± 0.2
1.25 ± 0.2
Table 3:
Constant of metMb binding to mitochondria at pH 6-8 and ionic strength ranging from 10 to 150 mM KCl.
