A. Circular dichroism analysis of HC27 oligonucleotide in a gradient of pH buffers.
B. The transitional pH was determined by plotting the molar ellipticity at 288 nm wavelength of each sample at their respective pH buffers.
C. Cooling and melting curves of HC27 oligonucleotide at pH 4.5, 6.5 and 7 done at a rate of 2°C/7min, where the hysteresis of the cooling and melting curves can be overlapped.
D. Melting curves of HC27 in varying pHs. The molar ellipticity was monitored at 288 nm while increasing the temperature from 4°C to 95°C at a rate of 7 min/2°C.
E. A graph comparing melting temperatures of HC27 at varying pH derived from the melting curves using the Boltzmann sigmoidal equation.
F. 16% native PAGE at pH 4.5 of the HC27 and CmidT oligonucleotides at varying concentrations (0.2, 2 and 20 μM), a poly-thymidine oligonucleotide 27 bp in length, run along two molecular weight markers at 52 and 26 basepairs.
G. Cooling and melting curves of HC27 oligonucleotide at 5 or 50 μM concentration at 288 nM wavelength.
