A. A graphical representation of the band intensity of RT-PCR results. The bands in the poly-acrylamide gel analysis corresponding to the PCR amplification of the pPu/pPy tract were scanned in a densitometer, and the band intensities were graphed relative to the Input signal.
B. A poly-acrylamide gel analysis of PCR products. Exponentially proliferating U251 cells wereharvested for ChIP assay. Protein-DNA cross-linked samples were immunoprecipitated with the indicated antibodies or control IgG, the samples washed and its DNA extracted and purified for RT-PCR analysis. A radiolabelled primer was used to amplify the pPu/pPy tract of the HIF-1α promoter.
