Figure 5: Exposure of IMCE-Ras cells to 5-Fu or 5-Aza-dC has different effects on the size of CSC population. (A) Schematic strategy for treatment of IMCE-Ras cells with 5-FU and 5-Aza-dC. The 5 x 104 cells were seeded into each well of 6-well plates 24 hrs before treatment. The cells were treated with 20mM of 5-FU or 1 μM of 5-Aza-dC for 2 days and then the drugs were washed out. After 5 days of recovery, the surviving cells were evaluated for CSC population by in vitro sphere assay and tumorigenicity by soft-agar assay. (B) Sphere formation assay. SFE, sphere forming efficiency. (C) Soft-agar assay.