Injected cells |
Tumors/Injections |
Frequency of CSCa |
p-value |
5x105 |
5x104 |
1x104 |
2x103 |
500 |
Tumorsphere |
2/2 |
2/2 |
2/2 |
2/2 |
1/2 |
1/890 |
0.0013 |
Monolayer |
2/2 |
1/2 |
0/2 |
0/2 |
0/2 |
1/390,850 |
aThe limit-dilution calculation was based on a Poisson probability distribution: ln (y) = rx + ln (a), where y is the ratio of non–tumor-generating injections, x is the number
of cells injected, and r is the frequency of CSC; a is the y intercept and it was assumed that with no cells injected, no tumors are formed (therefore at 100% non–tumor
generation, a = 1). The frequency of CSCs (r) was calculated from the slope of line best fit to the data points [56,57]. |
