RT-qPCR analysis of 10 pooled animal (A) or vegetal (V) halves at late stage III (A) and stage V (B) stages of oogenesis with specific primers for CK2α; XWnt11, a known vegetally localized transcript; XWnt5, an animally enriched transcript. From left to right, histograms represent transcript number normalized by Odc for CK2α, XWnt11 and XWnt5a. This experiment was repeated three times for the CK2α and Odc, and twice for the Wnts with similar results.
