| |
Level |
K’ |
T |
(Rs) |
| A: pH of mobile phase |
| 2.8 |
-0.2 |
3.8 |
1.39 |
2.4 |
| 3 |
0 |
3.9 |
1.43 |
2.3 |
| 3.2 |
0.2 |
3.6 |
1.4 |
2.2 |
| Mean ± S.D (n=6) |
3.9 ± 0.2 |
1.4 ± 0.02 |
2.3 ± 0.1 |
| B: Flow rate (mL/min) |
| 0.6 |
-0.2 |
3.8 |
1.45 |
2.32 |
| 0.8 |
0 |
3.9 |
1.43 |
2.36 |
| 1.0 |
0.2 |
3.6 |
1.42 |
2.37 |
| Mean ± S.D (n=6) |
4.3 ± 0.2 |
1.4 ± 0.01 |
2.3 ± 0.0 |
| C: Percentage of water in mobile phase (V/V) |
| 35 |
-5 |
4.6 |
1.42 |
2.38 |
| 40 |
0 |
3.9 |
1.43 |
2.36 |
| 45 |
5 |
4.5 |
1.46 |
2.33 |
| Mean ± S.D (n=6) |
4.3 ± 0.0 |
1.4 ± 0.02 |
2.3 ± 0.0 |
| D: Wavelength (nm) |
| 220 |
-5 |
3.5 |
1.52 |
2.5 |
| 225 |
0 |
3.9 |
1.43 |
2.36 |
| 230 |
5 |
3.4 |
1.55 |
2.3 |
| Mean ± S.D (n=6) |
3.3 ± 0.1 |
1.5 ± 0.0 |
2.4 ± 0.1 |
K’=Capacity factors, N=Theoretical plates, T=Tailing factor Rs=Resolution |