Figure 4: Effects of THC on iNOS (A), TNF-α (B) and IL-1β (C) mRNA expression. Aggregate cultures remained either untreated [Ctrl] or were treated with THC (1 μM) [T]; IFN-γ (50 U/ml) plus LPS (5 μg/ml) [I+L]; or treated with THC simultaneously with the inflammatory agents [I+L+T]. These treatments were applied one time (1X) or three times (3X). For measuring the mRNA expression, cultures were harvested 24 hours after the inflammatory treatments. Values are expressed as fold change of untreated control cultures (=1), each value being the mean of 6-8 replicate cultures. Results were statistically evaluated for significance by the Kruskal-Wallis test followed by the Mann-Whitney test. (*P<0.05, **P< 0.01, ***P<0.001 compared with untreated control cultures; °°°P< 0.001 compared to cultures treated with the inflammatory agents; t = tendancy compared