Table 2. Frequency (%) of alterations (CNA, MN/B e AP/NE) observed in A. cepa meristematic cells exposed to the different concentrations of the Black Dye Commercial Product, and to the Negative and Positive Control Tests, for the 20, 48 and 72h treatments.

Treatment time		NC	PC	Black Dye Commercial Product Concentrations (mg/L)
Treatment time		NC	PC	1	10	100	1000
20 h treatment
CNA	GML	0.040 (0.011)	1.090 (0.013)ª	0.880 (0.023)	0.860 (0.021)	0.980 (0.019)	1.430 (0.021)ª
	CL	0.040 (0.011)	0.500 (0.021)	0.480 (0.017)	0.630 (0.018)ª	0.910 (0.026)ª	0.940 (0.013)ª
	CA	0.050 (0.012)	0.660 (0.024)ª	0.640 (0.019)ª	0.760 (0.010)ª	0.580 (0.014)ª	0.550 (0.014)ª
	CM	0.100 (0.016)	0.090 (0.016)	0.130 (0.015)	0.180 (0.016)	0.290 (0.018)	0.350 (0.016)
	PM	0	0.010 (0.000)	0.030 (0.001)	0.050 (0.001)	0.040 (0.001)	0.120 (0.002)
	PI	0	0.040 (0.001)	0.030 (0.001)	0.060 (0.002)	0.060 (0.002)	0.130 (0.003)
	CB	0.020 (0.008)	0.170 (0.019)	0.240 (0.014)	0.380 (0.016)ª	0.310 (0.021)	0.160 (0.012)
	MU	0	0.070 (0.002)	0.050 (0.001)	0.230 (0.010)	0.240 (0.010)	0.250 (0.011)
	CNA	0.250 (0.017)	2.630 (0.012)ª	2.480 (0.015)ª	3.150 (0.026)ª	3.410 (0.020)ª	3.930 (0.021)ª
MNB	MN	0.290 (0.013)	2.010 (0.014)ª	1.570 (0.017)ª	1.390 (0.013)ª	1.230 (0.017)	1.610 (0.014)ª
	CBr	0.050 (0.014)	0.690 (0.025)ª	0.310 (0.016)	0.190 (0.014)	0.560 (0.020)ª	0.390 (0.019)ª
	MNB	0.340 (0.013)	2.700 (0.014)ª	1.880 (0.017)ª	1.580 (0.013)ª	1.790 (0.017)ª	2.000 (0.014)ª
AP NE	AP	0	0	0.010 (0.004)	0	0	0.190 (0.012)ª
	NE	0	1.370 (0.023)ª	1.030 (0.020)ª	1.150 (0.021)ª	2.730 (0.035)ª	1.850 (0.024)ª
	APNE	0	1.370 (0.023)ª	1.040 (0.022)ª	1.150 (0.021)ª	2.730 (0.035)ª	2.040 (0.033)ª
TOTAL		0.590 (0.011)	6.700 (0.011)ª	5.400 (0.018)	5.880 (0.012)ª	7.930 (0.014)ª	7.970 (0.021)ª
48 h treatment
CNA	GML	0.190 (0.021)	1.200 (0.011)	0.390 (0.014)	0.380 (0.017)	0.320 (0.016)	0.320 (0.012)
	CL	0.090 (0.017)	1.030 (0.013)ª	1.000 (0.016)ª	0.600 (0.026)	0.430 (0.020)	0.880 (0.020)ª
	CA	0.170 (0.014)	0.540 (0.022)	0.660 (0.018)	0.780 (0.028)ª	0.730 (0.017)ª	0.650 (0.013)
	NB	0	0	0	0.110 (0.000)	0	0
	CM	0.120 (0.016)	0.380 (0.012)ª	0.390 (0.021)ª	0.260 (0.022)	0.220 (0.021)	0.330 (0.018)
	PM	0	0.080 (0.012)	0.130 (0.014)	0.080 (0.013)	0.100 (0.017)	0.090 (0.019)
	PI	0	0.100 (0.023)	0.160 (0.017)	0.140 (0.022)	0.150 (0.021)	0.140 (0.017)
	CB	0.030 (0.010)	0.270 (0.011)	0.260 (0.021)	0.550 (0.029)ª	0.410 (0.015)ª	0.320 (0.016)ª
	MU	0.050 (0.013)	0.060 (0.019)	0.120 (0.022)	0.180 (0.033)	0.210 (0.031)	0.140 (0.025)
	CNA	0.650 (0.019)	3.660 (0.012)ª	3.110 (0.012)ª	2.970 (0.019)ª	2.570 (0.013)	2.870 (0.011)ab
MNB	MN	0.440 (0.023)	1.730 (0.011)ª	1.260 (0.019)	0.760 (0.023)	1.660 (0.030)ª	1.470 (0.017)ª
	CBr	0.010 (0.008)	0.330 (0.009)ª	0.270 (0.023)ª	0.200 (0.026)	0.160 (0.031)	0.170 (0.022)
	MNB	0.450 (0.025)	2.060 (0.018)ª	1.530 (0.028)	0.960 (0.027)	1.820 (0.037)ª	1.640 (0.029)ª
AP NE	AP	0	0	0	0	0	0b
	NE	0	0.040 (0.023)b	0.020 (0.011)b	0.010 (0.015)b	0.030 (0.026)b	0.800 (0.029)ab
	APNE	0	0.040 (0.013)b	0.020 (0.005)b	0.010 (0.005)b	0.030 (0.009)b	0.800 (0.024)ab
TOTAL		1.100 (0.025)	5.760 (0.017)ª	4.660 (0.014)	3.940 (0.011)	4.420 (0.013)	5.310 (0.017)
72 h treatment
CNA	GML	0.040 (0.015)	0.740 (0.016)	0.220 (0.020)b	0.310 (0.023)	0.330 (0.018)	0.390 (0.026)
	CL	0.110 (0.016)	0.420 (0.033)	0.400 (0.019)	0.570 (0.027)	0.400 (0.023)	0.520 (0.036)b
	CA	0.100 (0.014)	0.150 (0.023)b	0.380 (0.025)	1.030 (0.029)ª	0.360 (0.016)	0.400 (0.030)
	CM	0.050 (0.027)	0.130 (0.019)	0.160 (0.022)	0.190 (0.024)	0.090 (0.015)	0.280 (0.025)
	PM	0	0.220 (0.034)	0.010 (0.029)	0.020 (0.012)	0.090 (0.019)	0.050 (0.021)
	PI	0.010 (0.012)	0.240 (0.025)	0.040 (0.022)	0.080 (0.028)	0.110 (0.034)	0.120 (0.022)
	CB	0.010 (0.024)	0.190 (0.025)	0.110 (0.036)	0.180 (0.023)	0.200 (0.055)	0.080 (0.013)
	MU	0.010 (0.027)	0.050 (0.051)	0.090 (0.042)	0.040 (0.016)	0.090 (0.035)	0.070 (0.032)
	CNA	0.330 (0.020)	2.140 (0.021)ª	1.410 (0.021)b	2.420 (0.020)ª	1.670 (0.017)ª	1.910 (0.019)ab
MNB	MN	0.440 (0.027)	1.350 (0.036)b	0.730 (0.027)b	0.520 (0.031)b	0.860 (0.023)b	0.550 (0.029)b
	CBr	0.010 (0.003)	0.160 (0.021)b	0.080 (0.013)b	0.060 (0.014)b	0.120 (0.004)b	0.040 (0.007)b
	MNB	0.450 (0.027)	1.510 (0.036)b	0.810 (0.027)b	0.580 (0.031)b	0.980 (0.024)b	0.590 (0.030)b
AP NE	AP	0	0	0.110 (0.014)b	0.040 (0.026)	0	0.010 (0.019)b
	NE	0.070 (0.023)	0.120 (0.021)b	0.100 (0.023)	0.200 (0.018)b	0.610 (0.022)ab	0.640 (0.026)ab
	APNE	0.070 (0.023)	0.120 (0.021)b	0.210 (0.013)b	0.240 (0.019)b	0.610 (0.022)ab	0.650 (0.029)ab
TOTAL		0.850 (0.021)	3.770 (0.037)ab	2.430 (0.018)b	3.240 (0.023)b	3.260 (0.029)b	3.150 (0.040)b

Note. NC. Negative Control; PC. Positive Control; CNA. Chromosome and nuclear aberrations; GML. Genetic material losses; CL. Chromosome losses; CA. Chromosome adherences; CM. C-metaphases; PM. Polyploidized metaphases; PI. Polyploidized interphases; CB. Chromosome bridges; MU. Multipolarities; MNB. Micronuclei and chromosome breaks; MN. Micronuclei; CBr. Chromosome breaks; APNE. Apoptotic and necrotic cells; AP. Apoptotic cells; NE. Necrotic cells; Total. Total of CNA, MNB and APNE. / 10,000 cells analyzed by concentration and by control. Average (Standard Deviation) / ªSignificantly different from the NC (p < 0,05) and b Significantly different from the 20h treatment (p < 0,05), according to the Kruskal-Wallis test.

Table 2: Frequency (%) of alterations (CNA, MN/B e AP/NE) observed in A. cepa meristematic cells exposed to the different concentrations of the Black Dye Commercial Product, and to the Negative and Positive Control Tests, for the 20, 48 and 72h treatments.