Treatment

Nucleolus per cell

NC

PC

Black Dye Commercial Product Concentrations (mg/L)

1

10

100

1000

 

20 h

1

  8.810 (0.042)

10.070 (0.042)

11.960 (0.035)

  7.190 (0.024)

18.430 (0.061)

10.240 (0.040)

2

47.560 (0.038)

47.030 (0.034)

56.300 (0.031)

32.710 (0.022)

39.390 (0.049)

43.680 (0.032)

3

40.470 (0.056)

34.480 (0.030)

27.860 (0.045)

43.550 (0.027)

34.300 (0.044)

36.480 (0.038)

4

 2.450 (0.061)

  7.250 (0.090)

  3.480 (0.098)

14.180 (0.127)   

  6.570 (0.058)

  7.380 (0.066)

5

 0.720 (0.027) 

  0.940 (0.046)

  0.350 (0.029)

  2.060 (0.037)

  1.190 (0.031)

  1.570 (0.051)

6

0

  0.160 (0.021)

  0.060 (0.019)

  0.160 (0.029)

  0.110 (0.034)

  0.530 (0.036)ª

7

0

  0.070 (0.001)

0

  0.110 (0.000)

0

  0.120 (0.003)

8

0

0

0

  0.040 (0.001)

0

0

9

0

0

0

0

0

0

10

0

0

0

0

0

0

 

48 h

1

  8.360 (0.045)

11.430 (0.032)

   8.050 (0.030)

   9.790 (0.028)

    6.580 (0.036)

   10.290 (0.043)

2

38.410 (0.029)

44.920 (0.038)

 37.130 (0.026)

 37.580 (0.031)

  24.980 (0.026)

   38.130 (0.039)

3

37.770 (0.040)

26.020 (0.051)

 43.790 (0.021)

 35.430 (0.036)

  43.060 (0.022)

   39.140 (0.046)

4

  14.780 (0.099)b

11.890 (0.202)

   9.530 (0.060)

 10.440 (0.090)

    22.150 (0.051)b

   11.320 (0.082)

5

  0.680 (0.198) 

  4.960 (0.040)

   0.980 (0.038)

   4.940 (0.036)ab

    2.990 (0.024)ab

  0.690 (0.027)

6

            0

  0.780 (0.032)a

  0.340 (0.011)ab

   1.780 (0.022)a

    0.230 (0.005)

  0.300 (0.033)

7

            0

            0

   0.170 (0.011)

0

0

  0.130 (0.024)

8

            0

            0

            0

0

0

0

9

            0

            0

            0

0

0

0

10

            0

            0

            0

      0.040 (0)

0

0

 

72h

1

13.390 (0.028)

16.150 (0.030)

12.670 (0.029)

   9.830 (0.025)

  15.200 (0.026)

    13.620 (0.024)

2

41.630 (0.023)

37.610 (0.024)

42.820 (0.028)

 26.260 (0.028)

  29.350 (0.021)

    30.270 (0.023)

3

37.640 (0.029)

38.030 (0.025)

36.650 (0.026)

 41.190 (0.020)

  36.360 (0.024)

    33.710 (0.020)

4

  6.120 (0.075)

  7.040 (0.045)

   6.170 (0.042)

  20.170 (0.027)a

    17.140 (0.032)ab

    19.760 (0.022)ab

5

  1.170 (0.031)

  0.850 (0.044)

 1.500 (0.032)b

  2.260 (0.061)

    1.730 (0.045)

      2.090 (0.024)

6

  0.050 (0.019)

  0.320 (0.021)

   0.110 (0.019)

  0.190 (0.029)

    0.170 (0.034)

      0.430 (0.035)

7

             0

0

   0.080 (0.002)

  0.040 (0.016)

    0.050 (0.013)

      0.120 (0.001)

8

             0

0

            0

           0

             0

                0

9

             0

0

            0

           0

             0

                0

10

             0

0

            0

     0.060 (0)

             0

                0

Note. NC. Negative Control; PC. Positive Control / 5,000 cells analyzed by concentration and by control. Average (Standard Deviation).
ªSignificantly different from the NC (p < 0,05) and  b Significantly different from the 20h treatment (p < 0,05), according to the Kruskal-Wallis test.

Table 3: Frequency (%) of nucleolus quantity observed in A. cepa meristematic cells exposed to the different concentrations of the Black Dye Commercial Product, and to the Negative and Positive Control Tests, for the 20, 48 and 72h treatments.