Drugs

Exposure Time (min)

DFIa

Apoptotic Cellsb

DNA Tailc ± SD

ATENOLOL

untreated

9,71

27

3,67 ±1,37

15

26,05

39

78,54 ± 11,64

30

33,99

43

83,90 ± 15,41

45

24,60

36

76,36 ± 10,38

ATORVASTATIN

untreated

7,00

18

7,73 ± 1,40

15

57,00

63

17,64 ± 4,63

30

70,00

83

49,85 ± 12,20

45

65,00

69

18,87 ± 5,21

OFLOXACIN

untreated

12,49

12

8,31 ± 0,78

15

39,82

54

37,87 ± 6,40

30

41,90

69

73,79 ± 10,26

45

40,85

67

47,23 ± 8,43

SILDENAFIL CITRATE

untreated

13,33

19

3,42 ± 2,17

15

33,30

31

43,30 ± 12,74

30

40,00

35

48,64 ± 17,43

45

56,66

46

54,61 ± 11,65

GEMFIBROZIL

untreated

5,30

16

4,94 ± 1,97

15

8,96

21

17,94 ± 10,71

30

10,32

35

22,38 ± 11,07

45

13,57

39

33,14 ± 15,48

IBUPROFEN

untreated

3,31

20

4,94 ± 1,97

15

6,66

49

14,93 ± 3,47

30

9,55

36

65,45 ± 14,73

45

4,05

32

34,93 ± 13,63

CARBAMAZEPINE

untreated

11,31

19

5,18 ± 1,04

15

26,60

25

11,78± 4,91

30

34,37

33

13,03  ± 6,32

45’

45,49

39

13,90 ± 6,90

DICLOFENAC

untreated

13,31

19

6,17 ± 2,62

15

29,81

21

12,13 ± 5,57

30

41,89

33

14,12 ± 5,34

45

46,06

42

23,35 ± 4,29

BEZAFIBRATE

untreated

16,66

16

8,31 ± 0,78

15

20,00

21

11,51 ± 5,98

30

32,50

27

33,90 ± 11,51

45

40,85

38

55,30 ± 10,87

aDNA Fragmentation Index expressed in percentage as a result of the TUNEL test
bNumber of apoptotic cells expressed in percentage as a result of the Diffusion Assay
cDNA present in the tail of the comet expressed in percentage after the COMET Assay SD = standard deviation

Table 2: Sperm DNA fragmentation values for each pharmacological compound tested in the present study.