 Long Evans rat pups were treated with 50 μl i.p. injections of saline (vehicle) or
2.0 mg/kg ethanol in saline on postnatal days 2, 4, 6, and 8. Rats were sacrificed
on P30 and cerebella were fixed and embedded in paraffin. Histological
sections were stained with Hematoxylin and Eosin. (A, B) Low (original, 40x)
and (C, D) high (original, 160x) magnification images demonstrating (A, B)
control cerebella with long slender folia with uniform thickness of the molecular
layer (ml), and well-populated Purkinje (pc) and granule cell (gc) layers in
cortex, compared with (C, D) ethanol-exposed cerebella, which had shallow,
blunted and simplified folia with irregular thickness of the molecular layer,
irregular thinning of the granule cell layer (gcl) and white matter (wm) cores,
and numerous gaps corresponding with loss of neurons in the Purkinje cell
layer. White lines span the thicknesses of the granule cell layers. |
