Figure 5: Fine histological features of chronic alcohol-induced liver disease and the effects of PPAR agonist treatments. Liver tissue from adult male rats that were chronically fed with (A-D) 0% or (E-H) 37% ethanol-containing liquid diets, and treated with (A,E) vehicle, or a (B,F) PPAR-α, (C,G) PPAR-δ, or (D,H) PPAR-γ agonist was embedded in Spurr’s epoxy resin. 1 μm thick sections were stained with Toluidine blue. Note uniform cytoplasm with minimal lipid droplets in (A-D) control and (G) ethanol+PPAR-δ agonist treated livers, and (E,F,H) prominent lipid droplets (greenish-gray, insets) and (E) mega-mitochondria (coarse cytoplasmic granularity) in ethanol-exposed livers. Original magnifications-400x; insets-1200x.