Figure 3: Identification of sites of metastasis in SUM149, SUM190, and MDA-IBC-3 preclinical xenograft models of IBC. Figure 3a & 3b: Luciferase-based images of the presence of metastatic lesions detected at 21-60 days following injection of SUM149 cells (5 x 106) tagged with a luciferase bioluminescent tag into mammary fat pads of NOD. Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice, identifying multiple sites of metastasis of SUM149 to scapula, humerus, tibia, and pelvis. Figure 3c: Images of the presence of lung metastasis following injection of SUM149, SUM190, MDA-IBC-3, and MDA-MB-231 cells that were tagged with a luciferase bioluminescent tag, detected by bioluminescent signal in cells within isolated lungs.