Figure 1: Agarose gel electrophoresis of the plasmid pKUM isolated from P.aeruginosa isolate1. The electrophoresis was conducted in 0.7% agarose gel and stained with 0.5 μg/mL ethidium bromide. The gel was then observed on the UV transilluminatior. The plasmid band was marked by arrow. Lane 1: P.aeruginosa isolate1 wild type, Lane 2: Plasmid pKUM in E.coli ATCC 25922(Rif r) transconjugants, Lane 3: Plasmid pKUM in E.coli transformants, Lane 4: Plasmid pKUM in kmr transformants, Lane 5: P. aeruginosa isolate1 cured derivative.