Table 3

Donor cells	Recipient cells	Selective medium	Conjugation frequency	phenotype Co-transferred
P. aeruginosa isolate1 PIT^r, CAZ^r, CPM^r, CTX^r, Gm^r, Km^r	E.coli ATCC 25922 (Rif^r)	MHA* + CTX(30µg/mL) Rif(100µg/mL) & Gm(30µg/mL) Rif(100µg/mL	3.13×10^-5 3.2 × 10-5	CAZ, Km

*MHA: Muller – Hinton agar.
A control for conjugation was carried out and it was 1 x 10^-9.
Conjugation was carried out by membrane filter technique with ratio of 2:1 for donor and recipient cells

Table 3: Conjugation between multiple drugresistance P.aeruginosa isolate 1 as donor and E.coli ATCC 25922(Rifr) as recipient by membrane filter method.