Figure 2: Impact of HBx mutant on transactivation activity. A, expression of HBx and artificial mutants constructed according to the naturally occurring mutation pattern of BCP in HepG2 cells 48 h after transfection. B, transactivation activity of HepG2 cotransfected with NF-κB-Luc or mtNF-κB-Luc construct plus the indicated HBx artificial mutant or empty vector pcDNA3.1. Xwt: expression vector containing wild-type X gene; Xmt-2, Xmt-3.1, Xmt-3.2 and Xmt-4: expression vector containing mutants X gene, bearing T1762/A1764 double mutant, V1753/A1764/T1762 triple mutant, T1762/A1764/A1768 triple mutant and V1753/T1762/A1764/A1768 quadruple mutant, respectively. *P<0.05; **P<0.01 vs. controls, such as pcDNA3.1 empty vector.