Figure 6: Consequences of gestational ethanol exposure on invasive trophoblasts. Long Evans dams fed with isocaloric liquid diets containing 0% or 8.2% (v/v) starting from E6, E10, E14, E15, or E16 and placental tissue with underlying mesometrium was harvested on E19. (A) Low magnification [original X 40] images of sections immunostained with cytokeratin antibody (CK) demonstrate the depth and distribution of invasive trophoblastic cells in (a) control and (b-f) ethanol-exposure groups. Reciprocal high magnification images [original X 400] (g-l) exhibit the distribution of invasive trophoblasts around the decidual arteries and within the mesometrialstroma. (B) CKpositive invasive trophoblasts within the mesometrium were quantified using unbiased stereology. Inter-group comparisons were made using ANOVA with Tukey’s multiple comparisons post-hoc test for statistical significance. N=9 (3 placentas/dam) per group.