Figure 1: Effects of chronic prenatal ethanol exposure on Wnt gene expression. Pregnant dams were chronically fed with isocaloric liquid diets containing 0%, 18%, or 37% ethanol by caloric content. Cerebella harvested from perinatal (P3), juvenile (P10), and young adolescent (P20) rats were used in probe-based qPCR amplification reactions to measure (A-C) Wnt5a and (D-F) Wnt5b expression. Results were normalized to β-actin which was measured simultaneously in the duplex qPCR reactions. Inter-group comparisons were made by two-way ANOVA (Table 2) with Tukey post-hoc tests (results shown in panels). N=8-10 cerebella/ group with each sample contributed from a different litter.